By cesarsed, Section News Posted on Mon Aug 20, 2007 at 01:48:23 AM PST
RADIOPROTECTION FOLLOWING ORAL DELIVERY OF
5,4'-DIHYDROXY-3,7-DIMETHOXYFLAVONE INSULT IN MURINE TEST SYSTEM
Raymond J. Sucgang*1 and Oliver Villaflores2
In cooperation with the
Chemists' & Educators' Support for the Advancement
of Relevant Science Education, Inc., CESARSED, Inc.
1R.J. Sucgang Center for Research in the Natural Sciences
Napti, Batan, Aklan
There are numerous explorations for natural products which potentially provide safeguards to damaging effects of ionizing radiation. In relation to radiotherapy, prospects exist for screening and evaluating plant metabolites which put together efforts of applying relatively higher doses of radiation sensible, so as to favor further mortification of tumor cells than normal. In the present study, the challenge is to assess the latent radioprotective potential of 5,4'-dihydroxy-3,7dimethoxyflavone insult in the murine test system. Yellow needles of 5,4'-dihydroxy-3,7-dimethoxyflavone were isolated from ethanolic extracts of red ginger, Alpinia purpurata, via series of column chromatography with suitable solvent systems. Identification and elucidation of the structure was achieved using combined techniques of IR, MS and NMR spectral methods. Radioprotective efficacy was evaluated by measuring the ability of 5,4'-dihydroxy-3,7-dimethoxyflavone insult to inhibit irradiation-induced injury in mice. Total white cell counts and hemoglobin were substantially depressed in Swiss albino male mice (5-6 weeks old) subsequent to 3Gy whole-body gamma irradiation. Lymphocyte counts in the irradiated group were consistently higher (54%) than in the non-irradiated group. Differences between groups in red blood cell counts are insignificant. Oral treatment of single doses of 5,4'-dihydroxy-3,7-dimethoxyflavone (1ug/kg body weight) 12 hours prior to 3Gy irradiation, reduced the experiential depression of white blood cells and hemoglobin counts by up to 10 % and 1% respectively. It is concluded that oral administration of 5,4'-dihydroxy-3,7-dimethoxyflavone at 1ug/kg body weight, confers significant systemic radioprotection to mice receiving acute whole-body gamma irradiation.
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